"Monolithically Integrated Semiconductor Fluorescence Sensor for Microfluidic Applications," Sensors and Actuators
Updated: Aug 12, 2014
Author(s): Evan Thrush, Ofer Levi, Laura J. Cook, Jason Deich, Andrea Kurtz, Stephen J. Smith, W.E. Moerner, James S. Harris Jr.
"This article presents a monolithically-integrated semiconductor sensor for fluorescence detection on a microfluidic platform. Vertical-cavity surface-emitting lasers (VCSELs) for 773 nm excitation, PIN photodetectors and optical emission filters have been integrated on one GaAs substrate. These optoelectronic components are optically coupled to a glass microfluidic channel (100 mwidth and 45mdepth) through the use of a discrete micro-lens to form a complete sensor. The experimental limit of detection was 250nM of IRDye 800 Phosphoramidite. Based on an S/N = 3, the theoretical limit of detection was determined to be 40 nM. Laser background levels currently limit the sensor sensitivity. Large gains in sensitivity are possible through the systematic reduction of laser background by increasing spectral and spatial filtration. The low-cost, compact and parallel architecture makes this sensor a candidate for fluorescence-based sensing applications."
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